牦牛胎儿皮肤毛囊的形态发生及E钙黏蛋白的表达和定位

doi:10.11843/j.issn.0366-6964.2016.02.025

Abstract

Abstract:

The objective of this study was to observe yak hair follicle morphogenesis and fetal hair follicle original sites，study the importance of E-cadherin (CDH1) in the development of hair follicles.Skin tissue sections of the Yak embryo head were observed，and the hair follicle morphogenesis was studied.The melanin granules of fetal skin at different month and the development of hair follicle original sites were detected.The expression of E-cadherin protein was analyzed by using immunohistochemistry.The mRNA transcription levels of E-cadherin gene in different month fetal head skin was tested by using qRT-PCR method.Results showed that the fetal head skin begins to form primary hair buds in embryonic age of 60-70 days，and form the hair bulbs structure in the embryonic age of 130 days.Secondary hair follicles differentiate out from primary hair follicles in gestational age of 80-90 days；The parts of the fetal skin melanin gathering situations in different periods were observed，the hair follicles differentiation was started from head，of which lower，eyebrows，eyelashes，and horn site were the most obvious；E-cadherin protein was located on the epidermis，dermis and hair follicles，and showed high expression in epidermis，and moderate positive expression in hair follicles.E-cadherin gene mRNA transcription were uptrend in different embryonic age of yak skin hair follicle，but was lower at the embryonic age of 90 days，it was obviously below those at 70 days，120 days and 130 days (P<0.05).Yak head hair follicle is developed in the embryonic age of 60-70 days to form hair buds，and hair follicle development is likely forms from head.E-cadherin gene is most likely involved in the development of yak hair follicles.

CLC Number:

S852.1

SHE Ping-chang，LIANG Chun-nian，PEI Jie，CHU Min，GUO Xian，YAN Ping. Fetal Skin Hair Follicle Morphogenesis and E-Cadherin Expression of the Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 397-403.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.02.025

https://www.xmsyxb.com/EN/Y2016/V47/I2/397

References

［1］张容昶.中国的牦牛［M］.兰州：甘肃科学技术出版社，1989.
ZHANG R C.China：The yak［M］.Lanzhou：Gansu Science and Technology Publishing House，1989.(in Chinese)
［2］HOLBROOK K A，MINAMI S I.Hair follicle embryogenesis in the human.Characterization of events in vivo and in vitro［J］.Ann NY Acad Sci，1991，642：167-196.
［3］DEVENPORT D，FUCHS E.Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles［J］.Nat Cell Biol，2008，10(11)：1257-1268.
［4］CHRISTIANO A M.Epithelial stem cells：stepping out of their niche［J］.Cell，2004，118(5)：530-532.
［5］SIEBER-BLUM M，GRIM M，HU Y F，et al.Pluripotent neural crest stem cells in the adult hair follicle［J］.Dev Dyn，2004，231(2)：258-269.
［6］MCELWEE K，HOFFMANN R.Growth factors in early hair follicle morphogenesis［J］.Eur J Dermatol，2000，10(5)：341-350.
［7］PAUS R，MÜLLER-RÖVER S，VAD DER VEEN C，et al.A comprehensive guide for the recognition and classification of distinct stages of hair follicle morphogenesis［J］.J Invest Dermatol，1999，113(4)：523-532.
［8］MOU C，JACKSON B，SCHNEIDER P，et al.Generation of the primary hair follicle pattern［J］.Proc Natl Acad Sci U S A，2006，103(24)：9075-9080.
［9］ANDL T，REDDY S，GADDAPARA T，et al.WNT signals are required for the initiation of hair follicle development［J］.Dev Cell，2002，2(5)：643-653.
［10］BOTCHKAREV V A，KISHIMOTO J.Molecular control of epithelial-mesenchymal interactions during hair follicle cycling［J］.J Investig Dermatol Symp Proc，2003，8(1)：46-55.
［11］LARUE L，OHSUGI M，HIRCHENHAIN J，et al.E-cadherin null mutant embryos fail to form a trophectoderm epithelium［J］.Proc Natl Acad Sci U S A，1994，91(17)：8263-8267.
［12］PERL A K，WILGENBUS P，DAHL U，et al.A causal role for E-cadherin in the transition from adenoma to carcinoma［J］.Nature，1998，392(6672)：190-193.
［13］KUPHAL S，BOSSERHOFF A K.E-cadherin cell-cell communication in melanogenesis and during development of malignant melanoma［J］.Arch Biochem Biophys，2012，524(1)：43-47.
［14］YOUNG P，BOUSSADIA O，HALFTER H，et al.E-cadherin controls adherens junctions in the epidermis and the renewal of hair follicles［J］.EMBO J，2003，22(21)：5723-5733.
［15］SCHMIDT-ULLRICH R，PAUS R.Molecular principles of hair follicle induction and morphogenesis［J］.Bioessays，2005，27(3)：247-261.
［16］NOWAK J A，POLAK L，PASOLLI H A，et al.Hair follicle stem cells are specified and function in early skin morphogenesis［J］.Cell Stem Cell，2008，3(1)：33-43.
［17］ADELSON D L，CAM G R，DESILVA U，et al.Gene expression in sheep skin and wool (hair)［J］.Genomics，2004，83(1)：95-105.
［18］GAT U，DASGUPTA R，DEGENSTEIN L，et al.De novo hair follicle morphogenesis and hair tumors in mice expressing a truncated β-catenin in skin［J］.Cell，1998，95(5)：605-614.
［19］LIU B，CUI Y，YANG B，et al.Morphometric analysis of yak placentomes during gestation［J］.Anat Rec(Hoboken)，2010，293(11)：1873-1879.
［20］NGANVONGPANIT K，MÜLLER H，RINGS F，et al.Targeted suppression of E-cadherin gene expression in bovine preimplantation embryo by RNA interference technology using double-stranded RNA［J］.Mol Reprod Dev，2006，73(2)：153-163.
［21］SLOMINSKI A，TOBIN D J，SHIBAHARA S，et al.Melanin pigmentation in mammalian skin and its hormonal regulation［J］.Physiol Rev，2004，84(4)：1155-1228.
［22］MATSUZAKI T，YOSHIZATO K.Role of hair papilla cells on induction and regeneration processes of hair follicles［J］.Wound Repair Regen，1998，6(6)：524-530.
［23］PAUS R，COTSARELIS G.The biology of hair follicles［J］. N Engl J Med，1999，341(7)：491-497.
［24］HARDY M H，LYNE A G.The pre-natal development of wool follicles in Merino sheep［J］.Aust J Biol Sci，1956，9(3)：423-441.
［25］张燕军，尹俊，李长青，等.内蒙古阿尔巴斯绒山羊胎儿期皮肤毛囊发生发育规律研究［J］.畜牧兽医学报，2006，37(8)：761-768.
ZHANG Y J，YIN J，LI C Q，et al.Study on development of skin and hair follicle from fetal Inner Mongolian Arbas cashmere goats［J］.Acta Veterinaria et Zootechnica Sinica，2006，37(8)：761-768.(in Chinese)
［26］KISHIMOTO J，BURGESON R E，MORGAN B A.Wnt signaling maintains the hair-inducing activity of the dermal papilla［J］.Genes Dev，2000，14(10)：1181-1185.
［27］MÜLLER-RÖVER S，TOKURA Y，WELKER P，et al.E-and P-cadherin expression during murine hair follicle morphogenesis and cycling［J］.Exp Dermatol，1999，8(4)：237-246.
［28］GAY D L，YANG C C，PLIKUS M V，et al.CD133 expression correlates with membrane beta-catenin and E-cadherin loss from human hair follicle placodes during morphogenesis［J］.J Invest Dermatol，2015，135(1)：45-55.
［29］JAMORA C，DASGUPTA R，KOCIENIEWSKI P，et al.Links between signal transduction，transcription and adhesion in epithelial bud development［J］.Nature，2003，422(6929)：317-322.

[1]	ZHU Jiaqiao, CHENG Laiyang, CAO Jiangqin, ZHU Min, LI Junwei, JU Huimin, LIU Zongping. Preliminary Study on the Location and Function of XRCC1 in Oocyte and Early Embryos [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2126-2133.
[2]	FANG Yingdong, CHENG Cuicui, LU Jia, CHENG Yujiao, ZHANG Rui, LI Peixuan, WANG Wenhui. Distribution Characteristics of Plasma Cells Secreting IgA and IgG in Rabbit Nasal Cavity [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2751-2762.
[3]	ZHANG Qian, CUI Yan, YU Sijiu, HE Junfeng, PAN Yangyang, WANG Meng. Distribution of Immune Cells in the Epididymis and Vas Deferens of Yaks (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1925-1933.
[4]	HAN Yawen, LAI Zhenlin, ZHANG Mengmei, ZHAO Huiying, LIU Tengfei. Effect of 4℃ Liquid Storage on Ultrastructure of Dairy Goat Spermatozoa [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 964-971.
[5]	ZHANG Yiling, KAN Zifei, NIU Zheng, YU Qiuhan, RAN Ling, ZHANG Shujuan, ZOU Hong, XU Shasha, ZHANG Jingyi, SONG Zhenhui. Preparation and Application of Precision-Cut Tissue Slices [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 339-348.
[6]	ZHANG Qian, CUI Yan, YU Sijiu, LIAO Bo, ZOU Shengnan, BAI Xuefeng, Seth Yaw Afedo, ZHAO Pengfei, LONG Min. Histological of Hair Follicles from Different Parts of Skin and Expression of TGF-β2 and HIF-1α in Young Yak (Bos grunniens) [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3224-3233.
[7]	ZHANG Zhidong, RYAN Eoin, LI Yanmin. Quantitative Analysis of FMDV Receptor Integrin mRNA Levels in Ovine Tissues at Different Developmental Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2626-2632.
[8]	REN Yuxin, CUI Yan, ZOU Shengnan, BAI Xuefeng, YU Sijiu, HE Junfeng, LIAO Bo. Histological Observation of Blood Vessels and Nerves and Expression of HIF-1α in Adult Yaks Skin [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2633-2641.
[9]	LI Yuejiao, CUI Yan, ZHANG Qian, HE Junfeng. Study on the Distribution and Expression of CTGF and FGF-2 in the Lungs of Yaks at Different Ages [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2025-2033.
[10]	CHEN Jiaqi, ZHANG Xiaodi, GU Zhaobing, Lü Longbao, WU Dongwang, WANG Rongjiao, MAO Huaming. Effects and Mechanism of Faecal Microbiota Transplantation on Intestinal Barrier Function in Piglets [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1195-1207.
[11]	ZHANG Chen, FENG Luqiu, LIN Hailan, HUANG Ziqing, WANG Naixiu, GAN Ling. Association Analysis of Differentially Expressed miRNAs and Transcriptomics in Piglet Hippocampus under Oxidative Stress [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1396-1406.
[12]	DU Xiaohua, MI Xiaoyu, WANG Haifang, WEN Yongqiang, LI Qiao, LIU Xia. Expression and Localization of Neuroglobin and Hypoxia Inducible Factor-1α in Yak's Hindbrain [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 789-798.
[13]	KONG Fuli, LIU Ranran, ZHAO Guiping, WEN Jie. Research Progress on Wooden Breast and White Striping Myopathies in Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2329-2340.
[14]	WANG Yali, CUI Yan, WANG Ji. Analysis of Casts of the Kidney Collection System Anatomical Structure in the Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2584-2589.
[15]	BAI Xuefeng, CUI Yan, YU Sijiu, LI Hui, HE Junfeng, LIAO Bo. Effect of HIF-1α on Renal Tubular Epithelial-to-Mesenchymal Transition in Yak [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 620-629.

Fetal Skin Hair Follicle Morphogenesis and E-Cadherin Expression of the Yak

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments